The zoo encouraged runners to get their kit off in a bid to raise more than £40,000
Nearly 200 runners who took part raised an incredible £28,000 for the big cats last night.
All funds raised will go towards the Zoological Society London’s conservation work in protecting the endangered felines.
Sumatran tigers are currently at severe risk from deforestation and poachers in Indonesia.
People were encouraged to cover themselves in their own stripes or don a tiger costume.
The zoo had closed to visitors before the risque runners took to the starting line.
Outlining the rules, the zoo said on its website: “Nudity is encouraged for all participants.”